Zeta PlusTM,the System of Choice for Single-use Depth Filtration
Features and Benefits:
Ergonomically designed holder system:
Large holder (model 16EZB and 16EZC) can be pivoted between horizontal and vertical positions
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Enables loading and unloading at waist height, easy and convenient to use
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Minimal fluid spills when handling spent capsules
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Single or two-stage depth filtration within one filter holder
Vertical flow path
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Full utilisation of the filter media
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Small footprint during filtration
Innovative capsule/manifold design:
Translucent plastic shell
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Easy detection of the liquid level inside,providing real time monitoring of thefiltration process
Solid core design with fully encapsulated shell
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Eliminates the need for a stainless steel housing and the cleaning step after filtration
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Low hold-up volume
Self guiding locking mechanism
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Fast, reliable and robust capsule-to-capsule connectivity
Lenticular style capsule design
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Linear scalability from small to large system
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Consistency between single-use and conventional depth filtration
Zeta Plus™ Filter Media:
●Both double-layer EXT and single-layer Zeta Plus™ filter media are available
● Superior performance in throughput and filtration efficiency
The Zeta Plus™ Encapsulated System is a specially designed depth filtration system for the bioprocessing industry where upstream cell culture clarification and impurity removal or downstream impurity reduction is required.
The small system is ideal for lab scale production or scale up studies and the large system is designed for production scale biomanufacturing. Each system is comprised of a filter holder, top and bottom manifolds and the required number of capsules.
The Zeta Plus Encapsulated Systems for production scale biomanufacturing feature an ergonomically designed large holder (model 16EZB and 16EZC) that can be pivoted between horizontal and vertical positions, allowing for convenient loading and unloading, minimal footprint during filtration, minimal fluid spills during unloading and full utilisation of the filter media.
The capsule design features a translucent plastic shell for easy liquid level detection. A self guiding locking mechanism enables fast and robust capsule-to-capsule connection and the industry leading 3M Purification Zeta PlusTM depth filter media provides superior filtration performance.
* Based on testing with 1M NaOH and 5% NaClO (Bleach).
Ask for Chemical Compatibility Guide for more information.
High Performance Filter Media
The Zeta PlusTM Encapsulated System utilises the industry leading Zeta Plus depth filter media, including the double layer filter media and single layer media options. It is the best choice for fluid clarification filtration in the biologics industry.The Zeta PlusTM EXT Series is a family of advanced dual zone depth filters designed to provide optimal clarification of bioprocess and biological fluids. The Zeta PlusTM EXT Series filter media consist of two distinct layers or “zones” of filter media with the upstream zone more open than the downstream one. This structure enhances the contaminant holding capacity of the filter media, reduces premature plugging and helps extend service life. All Zeta PlusTM EXT media grades are available in single-use format including a special grade (DEL108A) that is designed for lipid removal.
Applications
●Clarification of mammalian cell culture harvest
● Enhanced protection of downstream processes
● Clarification of bacteria, yeast and insect cell lysates
● Host Cell Proteins (HCPs) reduction
● Viruses and DNA reduction
● Protein aggregates reduction
● Endotoxin reduction
● Anti-foaming agents reduction
The primary application for the Zeta PlusTM Encapsulated System is the cell culture clarification after fermentation. The System can be employed alone or in combination with centrifugation or Tangential Flow Filtration (TFF). An alternative application for the Zeta PlusTM Encapsulated System is downstream impurity reduction. Taking advantage of its positive charge capacity, Zeta PlusTM filter media has been shown to be effective in removing contaminants such as host cell proteins (HCP), viruses, DNA, protein aggregates and endotoxins.
In monoclonal antibody production, Zeta PlusTMEXT Series filter media is not only used before protein chromatography column to improve the fluid clarification and prolong the service life of the column, but alsoincreasingly being used as a polishing step after Protein A column capture step, replacing traditional anion exchange chromatography.
Ergonomically designed filter holders
Traditional depth filtration systems utilise lenticular style cartridge filters and a vertical filtration fl ow path to allow easy access to process liquids and efficient utilisation of filter media. However, stacking cartridges from bottom to top can be cumbersome and dismantling used cartridges is often labour intensive. Furthermore, fluid spills during the unloading process could be problematic and, in some instances, might expose the operators to the process fluids.
Recognising the need for a depth filtration system that is single-use, scalable easy to use, 3M Purification designed filter holders (Model 16EZB and 16EZC) that can be pivotedbetween the horizontal position for loading and unloading the capsules and manifolds and the vertical position for filtration. Allowing loading andunloading at waist height eliminates the need for operators to lift capsules above their heads and reduces the risk of fluid spills when handling usedcapsules. The use of the vertical fl ow path allows for full media utilisation and a small system footprint during filtration.
Small holder (model 16EZA)
The small holder is available for scale-up studies and lab-scale protein production. This holder can accommodate from one to four 0.23m² capsules or one 1.6m² (double-layer EXT) or 2.5m² (single-layer) capsule. Either single-stage or two-stage depth filtration can be performed within the same holder. The standard small holder has a built-in torque limiter that will signal that the holder assembly is properly sealed. A modified version is available that is ideal for applications where autoclaving is required.
Large holder (model 16EZB)
The large holder can accommodate up to 17.5m² of single-layer Zeta Plus
™ filter media (or 11.2m² of double-layer Zeta Plus filter media) and is ideal for small to large production scale purification processes. Large holder is designed to be suitable for single-stage filtration and two-stage filtration within the same holder. None holder component will directly contact with the process fluid, thus avoiding the need for in-situ clean in place (CIP) and related verification after filtration.
Integrated system (model 16EZC）
The integrated system combines groups ofpivoted holder to meet larger volume biologicsproduction. The holder can be pivoted between horizontal and vertical positions by the built-in mechanism of the system.
Innovative capsule/manifold design
Two capsule sizes are available, a small or a large capsule which can be used with the Zeta Plus
TM Encapsulated System. The 0.23m²small capsule is designed for use with the small holder in lab-scale production and scale-up studies. The larger capsule is designed for use with both the small and large holders. It accommodates 1.6m² double-layer media or 2.5m² single-layer media. The larger capsule has a self guiding locking mechanism for a fast, easy and robust capsule-to-capsule connection and a solid core design that eliminates the need for stainless steel bands and centre post. In addition, this capsule also has two handles for convenient loading and unloading and a low capsule fill volume and post-blown down hold-up volume. The capsule plastic shells are available in two different materials for a broad range of applications. The standard capsules use polycarbonate, thus have the translucent plastic shells that enables easy liquid level detection. The alkaline resistant capsules use polyphenylene/polystyrene which can be used in applications where NaOH is required for sanitisation.Correspondingly, the single use top and bottom manifolds are also available in these two materials. The manifold set ensures the entire filtration process is fast, easy and clean. An extra pair of manifolds is required to perform twostage filtration within one holder assembly.
Figure 2: Nominal retention ratings for Zeta Plus™ grades
Scalability
The Zeta Plus Encapsulated System retains the lenticular filter design and vertical flow path that are characteristics of traditional depth filtration systems, helping ensure consistent performance from bench-scale to production scale and from traditional non-disposable to disposable systems.
Four different surface areas disposable scale-up devices are available for media grade selection and filter sizing. The BC25 capsule with 25cm² filter is ideal for media grade screening. The scale-up capsule filters with 170 cm², 340 cm² and 1020 cm² are designed for intermediate scale-up studies and lab-scale protein production. Combined with these small-scale devices, the Zeta Plus
TM Encapsulated System offers a completely single-use solution to process from 0.5 litre to over 2500 litre of liquid.
Notes:
1. All Zeta Plus™ EXT grades are available for order.
2. For further information regarding Zeta Plus™ EXT Series filter media,please contact your local 3M Purifi cation representative.
3. DEL108A is a Zeta Plus™ EXT grade with two single layers of Delipid (DEL1) media.
4. Single layer 05SP media is offered in 0.23m² and 1.6m² Capsule format. One part number example is E16E07A05SP.
Zeta PlusTMEncapsulated Capsule Filter Ordering Information
Guochu Technology (Xiamen) provides products of 3M, including: Zeta Plus™ Depth Filter Cartridge, Polisher ST Series Chromatography Filter, Harvest RC Series Filter, Emphaze™ AEX Series Chromatography Filter, LifeASSURETM PDA Filter Cartridge, LifeASSURE™ PFS Filter Cartridge, LifeASSURE™ BDA Filter Cartridge, LifeASSURE™ BA Series Filter Cartridge, LifeASSURE™ BNA Filter Cartridge, BetafineT™ DP Filter Cartridge, BetafineT™ XL Filter Cartridge, BetafineT™ BT Filter Cartridge, Micro-Klean™ RT Filter Cartridge, Micro-Klean™ D Filter Cartridge, Betapure™ AU Filter Capsule, Betapure™ NT-T Filter Capsule, 3M™ High Flow Filter Cartridge, 3M™ NB Filter Bag, Micro Screen™ Metal Filter Cartridge, Liqui-Cel™ Membrane Contactor, Liqui Flux™ B Cross-flow Membrane, 3M™ CFSI Activated Carbon Filter Cartridge, ZW Series Cartridge, Zeta Plus™ Series Cartridge, Sanitary High Flow Cartridge, etc.
For details, please contact +86-592-6518670, +86-592-6514970.
Guochu Technology (Xiamen) provides all models of 3M Zeta PlusTMDepth Filter Cartridge, including:
E16E01A05SP, E16E01A10SP, E16E01A30SP, E16E01A50SP, E16E01A60SP, E16E01A90SP, E16E01A30ZB, E16E01A60ZB, E16E01A90ZB, E16E01A120ZB, E16E01A30LP, E16E01A50LP, E16E01A60LP, E16E01A90L, E16E01A30L, E16E01A50LA, E16E01A60LA, E16E01A90LA, E16E01ADELI, E16E01ADELP, E16E07A05SP, E16E11A10SP, E16E11A30SP, E16E11A50SP, E16E11A60SP, E16E11A90SP, E16E11A30ZB, E16E11A60ZB, E16E11A90ZB, E16E11A120ZB, E16E11A30LP, E16E11A50LP, E16E11A60LP, E16E11A90LP, E16E11A30LA, E16E11A50LA, E16E11A60LA, E16E11A90LA, E16E11ADELI, E16E11ADELP, E16R01A05SP, E16R01A10SP, E16R01A30SP, E16R01A50SP, E16R01A60SP, E16R01A90SP, E16R01A30ZB, E16R01A60ZB, E16R01A90ZB, E16R01A120ZB, E16R01A30LP, E16R01A50LP, E16R01A60LP, E16R01A90LP, E16R01A30LA, E16R01A50LA, E16R01A60LA, E16R01A90LA, E16R01ADELI, E16R01ADELP, E16R07A05SP, E16R11A10SP, E16R11A30SP, E16R11A50SP, E16R11A60SP, E16R11A90SP, E16R11A30ZB, E16R11A60ZB, E16R11A90ZB, E16R11A120ZB, E16R11A30LP, E16R11A50LP, E16R11A60LP, E16R11A90LP, E16R11A30LA, E16R11A50LA, E16R11A60LA, E16R11A90LA, E16R11ADELI, E16R11ADELP, E16E01A05SP01A, E16E01A10SP02A, E16E01A30SP02A, E16E01A30SP03A, E16E01A60SP01A, E16E01A60SP02A, E16E01A60SP03A, E16E01A60SP05A, E16E01A90SP05A, E16E01A90SP08A, E16E01A60ZB05A, E16E01A90ZB05A, E16E01A90ZB08A, E16E01A120ZB05A, E16E01A120ZB08A, E16E01A120ZB10A, E16E01ADELI08A, E16E01ADELP08A, E16E07A05SP01A, E16E07A10SP02A, E16E07A30SP02A, E16E07A30SP03A, E16E07A60SP01A, E16E07A60SP02A, E16E07A60SP03A, E16E07A60SP05A, E16E07A90SP05A, E16E07A90SP08A, E16E07A60ZB05A, E16E07A90ZB05A, E16E07A90ZB08A, E16E07A120ZB05A, E16E07A120ZB08A, E16E07A120ZB10A, E16E07ADELI08A, E16E07ADELP08A, E16R01A05SP01A, E16R01A10SP02A, E16R01A30SP02A, E16R01A30SP03A, E16R01A60SP01A, E16R01A60SP02A, E16R01A60SP03A, E16R01A60SP05A, E16R01A90SP05A, E16R01A90SP08A, E16R01A60ZB05A, E16R01A90ZB05A, E16R01A90ZB08A, E16R01A120ZB05A, E16R01A120ZB08A, E16R01A120ZB10A, E16R01ADELI08A, E16R01ADELP08A, E16R07A05SP01A, E16R07A10SP02A, E16R07A30SP02A, E16R07A30SP03A, E16R07A60SP01A, E16R07A60SP02A, E16R07A60SP03A, E16R07A60SP05A, E16R07A90SP05A, E16R07A90SP08A, E16R07A60ZB05A, E16R07A90ZB05A, E16R07A90ZB08A, E16R07A120ZB05A, E16R07A120ZB08A, E16R07A120ZB10A, E16R07ADELI08A, E16R07ADELP08A, E0170FSA05SP, E0170FSA10SP, E0170FSA30SP, E0170FSA50SP, E0170FSA60SP, E0170FSA90SP, E0170FSA30ZB, E0170FSA60ZB, E0170FSA90ZB, E0170FSA120ZB, E0170FSA30LP, E0170FSA50LP, E0170FSA60LP, E0170FSA90LP, E0170FSA30LA, E0170FSA50LA, E0170FSA60LA, E0170FSA90LA, E0170FSADELI, E0170FSADELP, E0340FSA05SP, E0340FSA10SP, E0340FSA30SP, E0340FSA50SP, E0340FSA60SP, E0340FSA90SP, E0340FSA30ZB, E0340FSA60ZB, E0340FSA90ZB, E0340FSA120ZB, E0340FSA30LP, E0340FSA50LP, E0340FSA60LP, E0340FSA90LP, E0340FSA30LA, E0340FSA50LA, E0340FSA60LA, E0340FSA90LA, E0340FSADELI, E0340FSADELP, E1020FSA05SP, E1020FSA10SP, E1020FSA30SP, E1020FSA50SP, E1020FSA60SP, E1020FSA90SP, E1020FSA30ZB, E1020FSA60ZB, E1020FSA90ZB, E1020FSA120ZB, E1020FSA30LP, E1020FSA50LP, E1020FSA60LP, E1020FSA90LP, E1020FSA30LA, E1020FSA.50LA, E1020FSA60LA, E1020FSA90LA, E1020FSADELI, E1020FSADELP, E0170FSA05SP01A, E0170FSA10SP02A, E0170FSA30SP02A, E0170FSA30SP03A, E0170FSA60SP01A, E0170FSA60SP02A, E0170FSA60SP03A, E0170FSA60SP05A, E0170FSA90SP05A, E0170FSA90SP08A, E0170FSA60ZB05A, E0170FSA90ZB05A, E0170FSA90ZB08A, E0170FSA120ZB05A, E0170FSA120ZB08A, E0170FSA120ZB10A, E0170FSADELI08A, E0170FSADELP08A, E0340FSA05SP01A, E0340FSA10SP02A, E0340FSA30SP02A, E0340FSA30SP03A, E0340FSA60SP01A, E0340FSA60SP02A, E0340FSA60SP03A, E0340FSA60SP05A, E0340FSA90SP05A, E0340FSA90SP08A, E0340FSA60ZB05A, E0340FSA90ZB05A, E0340FSA90ZB08A, E0340FSA120ZB05A, E0340FSA120ZB08A, E0340FSA120ZB10A, E0340FSADELI08A, E0340FSADELP08A, E1020FSA05SP01A, E1020FSA10SP02A, E1020FSA30SP02A, E1020FSA30SP03A, E1020FSA60SP01A, E1020FSA60SP02A, E1020FSA60SP03A, E1020FSA60SP05A, E1020FSA90SP05A, E1020FSA90SP08A, E1020FSA60ZB05A, E1020FSA90ZB05A, E1020FSA90ZB08A, E1020FSA120ZB05A, E1020FSA120ZB08A, E1020FSA120ZB10A, E1020FSADELI08A, E1020FSADELP08A.
